SubtiBank SubtiBank
phoB [2018-02-13T12:39:03.000Z]
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phoB [2018-02-13T12:39:03.000Z]

alkaline phosphatase III, heptaprenylglyceryl phosphate processing phosphatase
locus
BSU05740
pI
5.89
mw
50.36 kDa
protein length
462 aa Sequence Blast
gene length
1386 bp Sequence Blast
function
aquisition of phosphate upon phosphate starvation, ether lipid synthesis
product
alkaline phosphatase III, heptaprenylglyceryl phosphate processing phosphatase
essential
no
ec
3.1.3.1
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    620,097 → 621,485

    The protein

    Catalyzed reaction/ biological activity

  • A phosphate monoester + H2O = an alcohol + phosphate (according to Swiss-Prot)

    • Protein family

  • alkaline phosphatase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|E0430C16C41440633647976A0F9183E24268E198|PhoA]

    • Modification

  • phosphorylated on ser/ thr/ tyr [Pubmed|16493705]

    • Structure

  • [PDB|3A52] (from ''Shewanella sp.'', 41% identity) [Pubmed|20057143]

    • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862]

    • Expression and Regulation

    Operons

    genes
    [gene|3B95AB3021280215B6B487A348D78FB5797AEEA6|phoB]-[gene|66D3E978E3DBA0410CA5597B5B860DF27C97335A|ydhF]
    description
    [Pubmed|10913081]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,16030210], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|9335276,10913081,16030210], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]

    • regulation

  • expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|9335276,10913081,16030210]
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    Biological materials

    Mutant

  • 1A767 ( ''phoB''::''cat''), [Pubmed|9426145], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A767&Search=1A767 BGSC]
  • BKE05740 (Δ[gene|3B95AB3021280215B6B487A348D78FB5797AEEA6|phoB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTGGATAACCTCCTAAA, downstream forward: _UP4_TAAAAGAATACAAGGTGTCC
  • BKK05740 (Δ[gene|3B95AB3021280215B6B487A348D78FB5797AEEA6|phoB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTGGATAACCTCCTAAA, downstream forward: _UP4_TAAAAGAATACAAGGTGTCC

    • References

  • 16030210,9335276,8088554,10913081,16491025,8113174,9335276,18957862,16493705,25666134,20057143,27118079,27226549